Faculty Publications

Double-Stranded RNA Isolated From Grapevines Affected by Rupestris Stem Pitting Disease

Document Type

Article

Keywords

rupestris stem pitting, double-stranded RNA, Legno-riccio, grapevine virus "A", grapevine stem pitting, Pinot noir, virus-free

Journal/Book/Conference Title

American Journal of Enology and Viticulture

Volume

42

Issue

3

First Page

175

Last Page

179

Abstract

A survey of healthy and rupestris stem pitting (SP) diseased grapevines has been made for the presence of double-stranded RNA (dsRNA). Commercially important Pinot noir and Pinot gris clones from Oregon State University (OSU) were selected on the basis of virus disease indexing results. Double-stranded RNA was extracted from leaves and dormant cane phloem of mature grapevines, purified by column chromatography, and characterized by RNase digestion, lithium chloride precipitation, acridine orange staining, and agarose gel electrophoresis. The single dsRNA species observed that was unique to SP diseased vines was approximately 5.5 x 106 daltons. All SP-diseased vines contained one or more species of dsRNA while virus-free tissues contained none. Antisera prepared against grapevine virus "A" and against grapevine "potyvirus-like virus" were used in preliminary enzyme linked immunosorbent assays and gel diffusion tests, but results were inconclusive. Uses of dsRNA analysis for detection of SP disease are discussed.

Department

Department of Biology

Original Publication Date

1-1-1991

DOI of published version

10.5344/ajev.1991.42.3.175

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