Faculty Publications

Large Scale Separation of Naphthyltetrahydroisoquinoline Alkaloids from Ancistrocladus korupensis by Centrifugal Partition Chromatography

Document Type

Article

Journal/Book/Conference Title

Journal of Chromatography A

Volume

688

Issue

1-2

First Page

83

Last Page

88

Abstract

Crude extracts of Ancistrocladus korupensis contain a complex mixture of naphthyltetrahydroisoquinoline alkaloids, including the human immunodeficiency virus-inhibitory dimeric alkaloids michellamines A and B and the antimalarial monomeric korupensamines A-D. The efficient separation of michellamines A and B from these extracts has been accomplished by centrifugal partition chromatography. The Chromatographic conditions used on a multi-channel cartridge unit (Sanki LLN) have been successfully scaled up with a newly developed, stacked-disk type centrifugal partition chromatography unit (Sanki NMF) for separating larger amounts of alkaloid mixtures with similar resolution. A refined, three-step process (solvent-solvent partitioning, centrifugal partition chromatography and HPLC) has been developed and applied to the scaled-up production of michellamine B for preclinical drug development.

Department

Department of Chemistry and Biochemistry

Original Publication Date

12-30-1994

DOI of published version

10.1016/0021-9673(94)00923-6

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