Faculty Publications
Approaches for Determining DNA Persistence Length Using Atomic Force Microscopy
Document Type
Article
Keywords
Atomic force microscopy, DNA mechanics, Persistence length, Thymidine analogs, Wormlike chain model
Journal/Book/Conference Title
Methods in Molecular Biology
Volume
1837
First Page
211
Last Page
256
Abstract
Atomic force microscopy (AFM) is widely used to image and study biological molecules. As an example, we have utilized AFM to investigate how the mechanical properties of DNA polymers depend on electrostatics and the strength of DNA base stacking by studying double-stranded DNA molecules incorporating several different neutral and charged base modifications. Here, we describe ten complementary approaches for determining DNA persistence length by AFM imaging. The combination of different approaches provides increased confidence and statistical reliability over existing methods utilizing only a single approach.
Department
Department of Chemistry and Biochemistry
Original Publication Date
1-1-2018
DOI of published version
10.1007/978-1-4939-8675-0_13
Recommended Citation
Peters, Justin P. and Maher, L. James, "Approaches for Determining DNA Persistence Length Using Atomic Force Microscopy" (2018). Faculty Publications. 6228.
https://scholarworks.uni.edu/facpub/6228
