Faculty Publications

Fourier-Transform Mass Spectrometry of Large (M/Z > 5,000) Biomolecules

Document Type

Book Chapter

Journal/Book/Conference Title

Fourier Transform Mass Spectrometry: Evolution, Innovation, and Applications

Volume

359

First Page

100

Last Page

115

Abstract

Recent experimental results demonstrate the high mass (>10,000 amu) capabilities of Fourier transform mass spectrometry, however the data reveal non-theoretical limits in the resolution at high mass. These advances can be attributed to the development of methods for coupling high pressure ion sources to the ultra-high vacuum FT-ICR analyser. Specifically, external ion sources permit the utilization of liquid matrix secondary ionization mass spectrometry for the desorption of large involatile, thermally labile biomolecules with ion detection by FT-ICR. Limitations in the mass resolution arise from the inability to effectively trap the ions and produce a coherent packet of ions for detection. The lack of spatial and phase coherence of the injected ions leads to the loss of the frequency domain signal. According to our model, a principle factor contributing to the lack of spatial and phase coherence is field inhomogeneities coupled with the kinetic energies of the ions along the Z-axis.

Department

Department of Chemistry and Biochemistry

Original Publication Date

11-23-1987

DOI of published version

10.1021/bk-1987-0359.ch006

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