Faculty Publications
Fourier-Transform Mass Spectrometry of Large (M/Z > 5,000) Biomolecules
Document Type
Book Chapter
Journal/Book/Conference Title
Fourier Transform Mass Spectrometry: Evolution, Innovation, and Applications
Volume
359
First Page
100
Last Page
115
Abstract
Recent experimental results demonstrate the high mass (>10,000 amu) capabilities of Fourier transform mass spectrometry, however the data reveal non-theoretical limits in the resolution at high mass. These advances can be attributed to the development of methods for coupling high pressure ion sources to the ultra-high vacuum FT-ICR analyser. Specifically, external ion sources permit the utilization of liquid matrix secondary ionization mass spectrometry for the desorption of large involatile, thermally labile biomolecules with ion detection by FT-ICR. Limitations in the mass resolution arise from the inability to effectively trap the ions and produce a coherent packet of ions for detection. The lack of spatial and phase coherence of the injected ions leads to the loss of the frequency domain signal. According to our model, a principle factor contributing to the lack of spatial and phase coherence is field inhomogeneities coupled with the kinetic energies of the ions along the Z-axis.
Department
Department of Chemistry and Biochemistry
Original Publication Date
11-23-1987
DOI of published version
10.1021/bk-1987-0359.ch006
Recommended Citation
Hanson, Curtiss D.; Castro, Mauro E.; Russell, David H.; Hunt, Donald F.; and Shabanowitz, Jeffery, "Fourier-Transform Mass Spectrometry of Large (M/Z > 5,000) Biomolecules" (1987). Faculty Publications. 5719.
https://scholarworks.uni.edu/facpub/5719
