Faculty Publications

Biodegradation of 2,4,5-trichlorophenoxyacetic acid in liquid culture and in soil by the white rot fungus Phanerochaete chrysosporium

Document Type

Article

Journal/Book/Conference Title

Applied Microbiology and Biotechnology

Volume

31

Issue

3

First Page

302

Last Page

307

Abstract

Extensive biodegradation of [14C]-2,4,5-trichlorophenoxyacetic acid ([14C]-2,4,5-T) by the white rot fungus Phanerochaete chrysosporium was demonstrated in nutrient nitrogen-limited aqueous cultures and in [14C]-2,4,5-T-contaminated soil inoculated with this fungus and supplemented with ground corn cobs. After incubation of [14C]-2,4,5-T with aqueous cultures of the fungus for 30 days, 62.0%±2.0% of the [14C]-2,4,5-T initially present was degraded to 14CO2. Mass balance analysis demonstrated that water soluble metabolites were formed during degradation, and HPLC and thin layer chromatography (TLC) of methylene chloride-extractable material revealed the presence of polar and non-polar [14C]-2,4,5-T metabolites. It was also shown that only ∼5% of the [14C]-2,4,5-T initially present in cultures remained as undegraded [14C]-2,4,5-T. In incubations composed of [14C]-2,4,5-T-contaminated soil, ground corn cobs, and 40% (w/w) water, 32.5%±3.6% of the [14C]-2,4,5-T initially present was converted to 14CO2 after 30 days of incubation. These results suggest that it may be possible to develop practical systems based on the use of this fungus to detoxify 2,4,5-T-contaminated water and soil. © 1989 Springer-Verlag.

Original Publication Date

9-1-1989

DOI of published version

10.1007/BF00258414

Link to Full Text

Comments?

Share

COinS