Isolate Specific Detection Of Mycorrhizal Fungi Using Genome Specific Primer Pairs

Authors

Jason D. Abbas, University of Northern Iowa
B. A.D. Hetrick, University of Northern IowaFollow
J. E. Jurgenson, University of Northern IowaFollow

Document Type

Article

Keywords

DNA sequencing, Gigaspora margarita, Glomus mosseae, Mycorrhiza, PCR, RAPD, Zygomycetes

Journal/Book/Conference Title

Mycologia

Volume

88

Issue

6

First Page

939

Last Page

946

Abstract

Random amplification of polymorphic DNA analysis was used to generate DNA fragments that are unique to isolates of two arbuscular mycorrhizal fungi, Glomus mosseae and Gigaspora margarita. Sequence analysis of these fragments allowed generation of primer pairs, and subsequent specific identification of these genomes even in the presence of competing genomic DNA's. This approach can be used to specifically detect the presence of these fungi in mixtures of spores or infected roots.

Department

Department of Biology

Original Publication Date

1-1-1996

DOI of published version

10.2307/3761056

Recommended Citation

Abbas, Jason D.; Hetrick, B. A.D.; and Jurgenson, J. E., "Isolate Specific Detection Of Mycorrhizal Fungi Using Genome Specific Primer Pairs" (1996). Faculty Publications. 4169.
https://scholarworks.uni.edu/facpub/4169