Faculty Publications

Title

Expression of Tri15 in Fusarium sporotrichiodides

Document Type

Article

Keywords

Deoxynivalenol, Feedback inhibition, Mycotoxin, Negative control, T-2 toxin Zinc finger protein

Journal/Book/Conference Title

Current Genetics

Volume

45

Issue

3

First Page

157

Last Page

162

Abstract

In the fungus Fusarium sporotrichioides, biosynthesis of trichothecene mycotoxins requires at least three genetic loci: a core 12-gene cluster, a smaller two-gene cluster, and a single-gene locus. Here, we describe the Tri15 gene, which represents a fourth locus involved in trichothecene biosynthesis. Tri15 is predicted to encode a Cys2-His2 zinc finger protein and is expressed in a manner similar to genes in the core trichothecene gene cluster. However, disruption of F. sporotrichioides Tri15 does not affect production of T-2 toxin, the major trichothecene produced by this fungus. This result suggests that Tri15 is not necessary for the production of toxin. Cultures with exogenously added T-2 toxin have high levels of Tri15 expression and no detectable expression of the trichothecene biosynthetic genes Tri5 and Tri6. The expression analysis is consistent with Tri15 being a negative regulator of at least some of the trichothecene biosynthetic genes. In F. graminearum, Tri15 has been mapped to linkage group 2 and is therefore unlinked to the main trichothecene biosynthetic gene cluster. © Springer-Verlag 2003.

Original Publication Date

3-1-2004

DOI of published version

10.1007/s00294-003-0467-3

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