"Expression Of Tri15 In Fusarium Sporotrichiodides" by Nancy J. Alexander, Susan P. McCormick et al.
 

Faculty Publications

Expression Of Tri15 In Fusarium Sporotrichiodides

Document Type

Article

Keywords

Deoxynivalenol, Feedback inhibition, Mycotoxin, Negative control, T-2 toxin Zinc finger protein

Journal/Book/Conference Title

Current Genetics

Volume

45

Issue

3

First Page

157

Last Page

162

Abstract

In the fungus Fusarium sporotrichioides, biosynthesis of trichothecene mycotoxins requires at least three genetic loci: a core 12-gene cluster, a smaller two-gene cluster, and a single-gene locus. Here, we describe the Tri15 gene, which represents a fourth locus involved in trichothecene biosynthesis. Tri15 is predicted to encode a Cys2-His2 zinc finger protein and is expressed in a manner similar to genes in the core trichothecene gene cluster. However, disruption of F. sporotrichioides Tri15 does not affect production of T-2 toxin, the major trichothecene produced by this fungus. This result suggests that Tri15 is not necessary for the production of toxin. Cultures with exogenously added T-2 toxin have high levels of Tri15 expression and no detectable expression of the trichothecene biosynthetic genes Tri5 and Tri6. The expression analysis is consistent with Tri15 being a negative regulator of at least some of the trichothecene biosynthetic genes. In F. graminearum, Tri15 has been mapped to linkage group 2 and is therefore unlinked to the main trichothecene biosynthetic gene cluster. © Springer-Verlag 2003.

Department

Department of Biology

Original Publication Date

3-1-2004

DOI of published version

10.1007/s00294-003-0467-3

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