Faculty Publications

The Complete Mitochondrial Genome Of The Red-Jointed Brackish-Water Fiddler Crab Minuca Minax (Leconte 1855) (Brachyura: Ocypodidae): New Family Gene Order, And Purifying Selection And Phylogenetic Informativeness Of Protein Coding Genes

Document Type

Article

Keywords

Crustacea, Mitophylogenomics, Selective pressure

Journal/Book/Conference Title

Genomics

Volume

113

Issue

1

First Page

565

Last Page

572

Abstract

Minuca minax is a semi-terrestrial crustacean that commonly lives in low salinity, riverine habitats along the shores of the eastern United States. This study reports, for the first time, the complete mitochondrial genome of M. minax. The AT-rich mitochondrial genome of M. minax is 15,937 bp in length and comprised of 13 protein-coding genes (PCGs), 2 ribosomal RNA genes, and 22 transfer RNA genes. A single 737 bp long intergenic space is assumed to be the D-loop. Most of the PCGs and tRNA genes are encoded in the L-strand. The gene order observed in the mitochondrial genome of M. minax is new although almost identical to that reported in confamiliar species. In all other confamiliar species to which M. minax is compared, the positions of the trnQ gene and the trnI gene are switched. KA/KS ratios calculated for all mitochondrial PCGs show values of <1, indicating that these PCGs are evolving under purifying selection. A maximum likelihood phylogenetic analysis (concatenated PCGs [n = 13], 15 species) supports the monophyly of the subfamilies Ocypodinae and Gelaminidae. Mitochondrial PCGs have enough phylogenetic information to reveal relationships supporting higher taxonomic levels within this family. The knowledge of a complete mitochondrial genome from the red-jointed brackish-water fiddler crab M. minax contributes to the better understanding of meta-population connectivity and the mechanisms involved in the adaptation of marine organisms to near-limnic conditions.

Original Publication Date

1-1-2021

DOI of published version

10.1016/j.ygeno.2020.09.050

Repository

UNI ScholarWorks, Rod Library, University of Northern Iowa

Language

en

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