Mitochondrial Genome Of Geomydoecus Aurei, A Pocket-Gopher Louse

Authors

Theresa A. SpradlingFollow
Alexandra C. Place, University of Northern IowaFollow
Ashley L. Campbell, University of Northern IowaFollow
James W. Demastes, University of Northern IowaFollow

Comments

First published in PLOS ONE, July 27 (2021), eISSN 1932-6203.

DOI: https://doi.org/10.1371/journal.pone.0254138

Document Type

Article

Publication Version

Published Version

Journal/Book/Conference Title

PLOS ONE

Volume

July 27, 2021

First Page

1

Last Page

13

Abstract

Parasitic lice demonstrate an unusual array of mitochondrial genome architectures and gene arrangements. We characterized the mitochondrial genome of Geomydoecus aurei, a chewing louse (Phthiraptera: Trichodectidae) found on pocket gophers (Rodentia: Geomyidae) using reads from both Illumina and Oxford Nanopore sequencing coupled with PCR, cloning, and Sanger sequencing to verify structure and arrangement for each chromosome. The genome consisted of 12 circular mitochondrial chromosomes ranging in size from 1,318 to 2,088 nucleotides (nt). Total genome size was 19,015 nt. All 37 genes typical of metazoans (2 rRNA genes, 22 tRNA genes, and 13 protein-coding genes) were present. An average of 26% of each chromosome was composed of non-gene sequences. Within the nongene region of each chromosome, there was a 79-nt nucleotide sequence that was identical among chromosomes and a conserved sequence with secondary structure that was always followed by a poly-T region. We hypothesize that these regions may be important in the initiation of transcription and DNA replication, respectively. The G. aurei genome shares 8 derived gene clusters with other chewing lice of mammals, but in G. aurei, genes on several chromosomes are not contiguous.

Department

Department of Biology

Original Publication Date

7-27-2021

Object Description

1 PDF File

DOI of published version

10.1371/journal.pone.0254138

Repository

UNI ScholarWorks, Rod Library, University of Northern Iowa

Copyright

©2021 The Authors. CC BY license.

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Language

en

File Format

application/pdf

Recommended Citation

Spradling, Theresa A.; Place, Alexandra C.; Campbell, Ashley L.; and Demastes, James W., "Mitochondrial Genome Of Geomydoecus Aurei, A Pocket-Gopher Louse" (2021). Faculty Publications. 1.
https://scholarworks.uni.edu/facpub/1