2026 Summer Undergraduate Research Program (SURP) Symposium
Location
Schindler Education Center, University of Northern Iowa
Presentation Type
Open Access Poster Presentation
Document Type
poster
Abstract
The Conservation Reserve Program (CRP) created habitat across the US to decrease erosion and increase wildlife populations in agricultural landscapes. Its utility for wildlife is the subject of ongoing research, both the restoration and incoming habitants. We investigated the population genetic diversity of the brown-belted bumble bees in CRP sites within the agricultural landscapes in Northeastern Iowa. We generated Single Nucleotide Polymorphism (SNP) data using Restriction-site Associated DNA sequencing (RADSeq) and compared fixation indices and heterozygosity across four common SNP filtering levels. Due to sudden inadequacy of the RADSeq protocol, the final product was not resulting in usable concentrations of DNA. In order to complete this project, protocol modification was required.
Start Date
31-7-2026 11:00 AM
End Date
31-7-2026 1:30 PM
Event Host
Summer Undergraduate Research Program, University of Northern Iowa
Faculty Advisor
Ai Wen
Department
Department of Biology
Copyright
©2026 Halle Hoefing, Michael Mellett, and Ai Wen
File Format
application/pdf
File Size
1.33 MB
Recommended Citation
Hoefing, Halle; Mellett, Michael; and Wen, Ai, "Revised Restriction-site Associated DNA Sequencing Protocol and Bioinformatic Analysis" (2026). Summer Undergraduate Research Program (SURP) Symposium. 1.
https://scholarworks.uni.edu/surp/2026/all/1
Revised Restriction-site Associated DNA Sequencing Protocol and Bioinformatic Analysis
Schindler Education Center, University of Northern Iowa
The Conservation Reserve Program (CRP) created habitat across the US to decrease erosion and increase wildlife populations in agricultural landscapes. Its utility for wildlife is the subject of ongoing research, both the restoration and incoming habitants. We investigated the population genetic diversity of the brown-belted bumble bees in CRP sites within the agricultural landscapes in Northeastern Iowa. We generated Single Nucleotide Polymorphism (SNP) data using Restriction-site Associated DNA sequencing (RADSeq) and compared fixation indices and heterozygosity across four common SNP filtering levels. Due to sudden inadequacy of the RADSeq protocol, the final product was not resulting in usable concentrations of DNA. In order to complete this project, protocol modification was required.
