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Thesis (UNI Access Only)

Keywords

Proteomics; Barley--Effect of drought on; Barley--Development;

Abstract

Abiotic stress is responsible for as much as 60% of annual crop loss worldwide. The most detrimental of the abiotic stresses is drought. Drought during grain-filling is especially harmful to crop yield. In addition to the male and female reproductive structures, the spike (inflorescence) of cereals contains non-reproductive, photosynthetic organs known as the glume, lemma, awn and palea. The lemma and palea form the husk (hull). The non-reproductive structures are highly photosynthetic and supply the developing grain with carbon and nitrogen. They are drought tolerant and also serve as protective structures. The role of the non-reproductive organs of the spike in grain-filling becomes more important during drought, when most leaves wilt and senesce, resulting in a significant loss in photosynthetic output. In this study, the proteome of the barley awn, lemma, palea and kernel were examined using two-dimensional polyacrylamide gel electrophoresis (2D-PAGE). The proteome is compared before, and during grain-filling for each organ, and across the four organs under normal (well-watered) conditions to examine differences in the type of proteins expressed when there is no stress. In addition, changes in the proteome were characterized for all organs at both developmental stages during drought stress. Results and discussions highlight the functions of the proteins that show differential expression in the spike organs under normal and drought conditions.

Date of Award

2014

Degree Name

Master of Science

Department

Department of Biology

First Advisor

Tilahun Abebe

Date Original

2014

Object Description

1 PDF file (x, 102 pages)

Language

EN

File Format

application/pdf

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