Cultivation of Mesophilic Soil Crenarchaeotes in Enrichment Cultures from Plant Roots

Authors

Holly M. Simon
Courtney E. Jahn
Luke T. Bergerud
Marek K. Sliwinski, University of Northern IowaFollow
Paul J. Weimer
David K. Willis
Robert M. GoodmanFollow

Document Type

Article

Journal/Book/Conference Title

Applied and Environmental Microbiology

Volume

71

Issue

8

First Page

4751

Last Page

4760

Abstract

Because archaea are generally associated with extreme environments, detection of nonthermophilic members belonging to the archaeal division Crenarchaeota over the last decade was unexpected; they are surprisingly ubiquitous and abundant in nonextreme marine and terrestrial habitats. Metabolic characterization of these nonthermophilic crenarchaeotes has been impeded by their intractability toward isolation and growth in culture. From studies employing a combination of cultivation and molecular phylogenetic techniques (PCR– single-strand conformation polymorphism, sequence analysis of 16S rRNA genes, fluorescence in situ hybridization, and real-time PCR), we present evidence here that one of the two dominant phylotypes of Crenarchaeota that colonizes the roots of tomato plants grown in soil from a Wisconsin field is selectively enriched in mixed cultures amended with root extract. Clones recovered from enrichment cultures were found to group phylogenetically with sequences from clade C1b.A1. This work corroborates and extends our recent findings, indicating that the diversity of the crenarchaeal soil assemblage is influenced by the rhizosphere and that mesophilic soil crenarchaeotes are found associated with plant roots, and provides the first evidence for growth of nonthermophilic crenarchaeotes in culture.

Department

Department of Biology

Comments

First published in Applied and Environmental Microbiology. v. 71 n. 8 (2005), pp. 4751-4760, published by American Society for Microbiology (ASM). DOI: 10.1128/AEM.71.8.4751-4760.2005

Original Publication Date

2005

DOI of published version

10.1128/AEM.71.8.4751-4760.2005

Repository

UNI ScholarWorks, University of Northern Iowa, Rod Library

Date Digital

2005

Copyright

©2005 American Society for Microbiology (ASM). The copyright holder has granted permission for posting.

Language

EN

File Format

application/pdf

Recommended Citation

Simon, Holly M.; Jahn, Courtney E.; Bergerud, Luke T.; Sliwinski, Marek K.; Weimer, Paul J.; Willis, David K.; and Goodman, Robert M., "Cultivation of Mesophilic Soil Crenarchaeotes in Enrichment Cultures from Plant Roots" (2005). Faculty Publications. 11.
https://scholarworks.uni.edu/bio_facpub/11