2023 Summer Undergraduate Research Program (SURP) Symposium
Location
ScholarSpace, Rod Library, University of Northern Iowa
Presentation Type
Open Access Poster Presentation
Document Type
poster
Abstract
DNA is the foundation of an organism's ability to develop, survive, and reproduce. High genomic DNA is imperative for many scientific studies and applications such as Biotechnology, Genetics, & Pharmaceuticals. The separation of genomic DNA once purified from other cellular components (RNA, protein) can be sequenced to study entire genomes, develop antibodies, and limit genetic mutations.
Although a very stable molecule, DNA is susceptible to degradation under normal cellular conditions to various factors. Hence it is essential to properly extract genetic material from bee specimens, to better understand their population genetics.
DNA often needs to be stored for various lengths of time. To produce reliable and reproducible results, stability without contamination needs to be ensured to prevent degradation. In this study the goal is to show the stability & difference in quality of DNA purified from wild bees using the QIAGEN DNeasy® Blood & Tissue kit.
Start Date
28-7-2023 11:00 AM
End Date
28-7-2023 1:30 PM
Event Host
Summer Undergraduate Research Program, University of Northern Iowa
Faculty Advisor
Ai Wen
Department
Department of Biology
Copyright
©2023 Joel Lado and Dr. Ai Wen
File Format
application/pdf
Recommended Citation
Lado, Joel and Wen, Ai, "Assessment of DNA Concentration and Degradation from Extracted Bumble Bee Thorax Muscle 2023" (2023). Summer Undergraduate Research Program (SURP) Symposium. 12.
https://scholarworks.uni.edu/surp/2023/all/12
Assessment of DNA Concentration and Degradation from Extracted Bumble Bee Thorax Muscle 2023
ScholarSpace, Rod Library, University of Northern Iowa
DNA is the foundation of an organism's ability to develop, survive, and reproduce. High genomic DNA is imperative for many scientific studies and applications such as Biotechnology, Genetics, & Pharmaceuticals. The separation of genomic DNA once purified from other cellular components (RNA, protein) can be sequenced to study entire genomes, develop antibodies, and limit genetic mutations.
Although a very stable molecule, DNA is susceptible to degradation under normal cellular conditions to various factors. Hence it is essential to properly extract genetic material from bee specimens, to better understand their population genetics.
DNA often needs to be stored for various lengths of time. To produce reliable and reproducible results, stability without contamination needs to be ensured to prevent degradation. In this study the goal is to show the stability & difference in quality of DNA purified from wild bees using the QIAGEN DNeasy® Blood & Tissue kit.
