Presidential Scholars Theses (1990 – 2006)

Awards/Availabilty

Open Access Presidential Scholars Thesis

First Advisor

James Jurgenson

Abstract

RNAse P is a ribonucleoprotein involved in cutting out pieces of RNA. Specifically, it is involved in tRNA processing as it makes a single endonucleolytic cleavage in all precursor tRNA's to produce mature 5' ends. The sequence encoding the RNA subunit (rnpB gene) of RNAseP has been previously determined in one strain of Chlorobium limicola. A second strain was obtained from Carolina Biological Supply to test for conservation of sequence characteristics. This second strain was used in a PCR with the primers described in Haas. The amplified rnpB gene sequence appeared to have fewer base· pairs than the Haas strain when analyzed against a template in gel electrophoresis. Restriction enzyme analysis of the amplified showed consistencies with the Haas sequence. Sequencing of the isolated gene confirmed the similarity between the the Carolina strain of RNAseP and the Haas strain. Structural differences were maintained.

Date of Award

1996

Department

Department of Biology

Presidential Scholar Designation

A paper submitted in partial fulfillment of the requirements for the designation Presidential Scholar

Comments

If you are the rightful copyright holder of this Presidential Scholars thesis and wish to have it removed from the Open Access Collection, please submit an email request to scholarworks@uni.edu. Include your name and clearly identify the thesis by full title and author as shown on the work.

Date Original

1996

Object Description

1 PDF file (25 pages)

Date Digital

2-22-2018

Copyright

©1996 - Emily J. Hoeger

Type

document

Language

EN

File Format

application_pdf

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