Open Access Presidential Scholars Thesis
RNAse P is a ribonucleoprotein involved in cutting out pieces of RNA. Specifically, it is involved in tRNA processing as it makes a single endonucleolytic cleavage in all precursor tRNA's to produce mature 5' ends. The sequence encoding the RNA subunit (rnpB gene) of RNAseP has been previously determined in one strain of Chlorobium limicola. A second strain was obtained from Carolina Biological Supply to test for conservation of sequence characteristics. This second strain was used in a PCR with the primers described in Haas. The amplified rnpB gene sequence appeared to have fewer base· pairs than the Haas strain when analyzed against a template in gel electrophoresis. Restriction enzyme analysis of the amplified showed consistencies with the Haas sequence. Sequencing of the isolated gene confirmed the similarity between the the Carolina strain of RNAseP and the Haas strain. Structural differences were maintained.
Date of Award
Department of Biology
Presidential Scholar Designation
A paper submitted in partial fulfillment of the requirements for the designation Presidential Scholar
1 PDF file (25 pages)
©1996 Emily J. Hoeger
Hoeger, Emily J., "Characterization of the RNaseP sequence in Chlorobium limicola" (1996). Presidential Scholars Theses (1990 – 2006). 85.