2021 Summer Undergraduate Research Program (SURP) Symposium

Location

Ballroom, Maucker Student Union, University of Northern Iowa

Presentation Type

Poster Presentation (UNI Access Only)

Document Type

poster

Abstract

A crucial step in the DNA repair mechanism of homologous recombination is the search for homology between DNA molecules which is closely followed by strand exchange between homologous DNA. This step is governed by the nucleoprotein RAD51 which competes with Replication Protein A (RPA) to saturate single-stranded DNA (ssDNA) overhangs. Such interactions are naturally stochastic, so a dynamic stochastic model is the best representation of the real molecular process occurring during homologous recombination. Forming a stochastic Monte Carlo model of the competition between RAD51 and RPA allows for the observation of real-time dynamics during these interactions. Such a dynamic model was developed in Matlab and is being used to study the effects that various governing parameters have on each protein’s saturation level on ssDNA.

Start Date

30-7-2021 11:30 AM

End Date

30-7-2021 1:15 PM

Event Host

Summer Undergraduate Research Program, University of Northern Iowa

Faculty Advisor

Ali Tabei

Department

Department of Physics

File Format

application/pdf

Off-Campus Download

Share

Import Event to Google Calendar

COinS
 
Jul 30th, 11:30 AM Jul 30th, 1:15 PM

Stochastic Monte Carlo Simulations of Competing Nucleoproteins on Single-Stranded DNA

Ballroom, Maucker Student Union, University of Northern Iowa

A crucial step in the DNA repair mechanism of homologous recombination is the search for homology between DNA molecules which is closely followed by strand exchange between homologous DNA. This step is governed by the nucleoprotein RAD51 which competes with Replication Protein A (RPA) to saturate single-stranded DNA (ssDNA) overhangs. Such interactions are naturally stochastic, so a dynamic stochastic model is the best representation of the real molecular process occurring during homologous recombination. Forming a stochastic Monte Carlo model of the competition between RAD51 and RPA allows for the observation of real-time dynamics during these interactions. Such a dynamic model was developed in Matlab and is being used to study the effects that various governing parameters have on each protein’s saturation level on ssDNA.