Open Access Presidential Scholars Thesis
A mouse gene cloned in the laboratory of James and Olivia Smith at Baylor College of Medicine was found to be involved in motor function. This gene was named CRFl for Clq-Related Factor 1, and using in situ hybridization, it was found to be expressed mainly in the brainstem of adult mice. There is a gene similar to the CRFl gene in humans. By determining this gene's role in mouse development, more will be known about the development of human motor function.
To determine the stage of embryonic development at which the expression of this gene begins, a series of in situ hybridization experiments were performed on saggital sections of embryos at 11.5, 12.5, 13.5, 14.5, and 16.5 days of development using a diglabeled riboprobe made from CRF template DNA. Two types of substrate detection, NBT/BCIP and fluorescence, were used to visualize the probe. In several experiments, no specific hybridization occurred. In the remainder of the experiments, the hybridization that occurred in specific tissues was not repeatable and therefore unreliable.
Date of Award
Department of Biology
Presidential Scholar Designation
A paper submitted in partial fulfillment of the requirements for the designation Presidential Scholar
1 PDF file (19 pages)
©1999 - Margaret Gruber
Gruber, Margaret, "The expression of the CRF1 gene in mouse embryonic development" (1999). Presidential Scholars Theses (1990 – 2006). 75.