ovarian Ketosteroids, 2, 4-Dinitrophenylhydrazine derivatives, steroid analysis
This paper reports the development of a technically simple analytical technique for the characterization and quantitative determination of ovarian ketosteroids employing derivative formation with 2,4-dinitrophenylhydrazine (2,4-DNP), separation by thin-layer chromatography (TLC), and quantitative determination by spectrophotometry. The chemical identities of the derivatives were established by nitrogen analysis. The melting point, chromatographic mobilities of the free steroids and their derivatives on silica gel G in three systems, wavelength of maximum absorption in chloroform and ethanol, and molar absorption coefficient are reported for the 2,4-DNP derivatives of ovarian ketosteroids. The relationship between the absorption maximum in chloroform and the chemical structure of the derivatives has been analyzed.
Proceedings of the Iowa Academy of Science
©1978 Iowa Academy of Science, Inc.
Black, Asa C. Jr.; Davenport, G. Rodman; and West, James R.
"The Use of 2,4-Dinitrophenylhydrazine Derivatives of Ovarian Ketosteroids in Steroid Analysis,"
Proceedings of the Iowa Academy of Science, 85(3), 99-102.
Available at: https://scholarworks.uni.edu/pias/vol85/iss3/8