Amino Acids, Nutrition, Acanthamoeba
Quantitative analyses of the amino acids in the culture media before and after population growth demonstrate that Acanthamoeba castellanii selectively utilizes amino acids from Adam's defined medium (DM2) and Neff's optimal growth medium, but no amino acid is completely depleted during normal population growth. The cellular amino acid content varies for amoebae grown in the two media. The most common total cellular amino acids are alanine, aspartic acid, glutamic acid, glycine, and leucine. The most common free amino acids in the intracellular pools are alanine and proline. In DM2, NH3 is produced at a rate of 0.027 picomoles/ amoebae/hour.
Proceedings of the Iowa Academy of Science
©1976 Iowa Academy of Science, Inc.
Dolphin, Warren D.
"Nitrogen Metabolism in Acanthamoeba castellanii: Amino Acid Consumption and Composition Patterns,"
Proceedings of the Iowa Academy of Science, 82(3-4), 171-174.
Available at: https://scholarworks.uni.edu/pias/vol82/iss3/9