Thyroxine, chick limb bud, ectoderm-meso-derm interaction, RNA, acid phosphatase
Transplanted hind limb buds of chick embryos at stage18 were treated with thyroxine, which caused complete growth inhibition at concentrations of 200, 150, and 100 μg/ml. Lower concentrations caused reduction in length and weight as well as decreased number of feather germs. An increase in the enzyme acid phosphatase and number of lysosomes, in the cells of limb buds, were also observed. Increased acid phosphatase reaction was accompanied by decreased amounts of RNA (in treatment with 50 and 70 μg/ml. concentrations), suggesting a correlation between the two thus showing a thyroxine effect on lysosome activity.
Proceedings of the Iowa Academy of Science
©1973 Iowa Academy of Science, Inc.
Suleiman, Suleiman A.
"Effect of Thyroxine on Early Development of Chick Limb Buds,"
Proceedings of the Iowa Academy of Science, 80(3), 140-144.
Available at: https://scholarworks.uni.edu/pias/vol80/iss3/8