That many of the fixing reagents in common use in the preparation of tissues for histological study are poorly adapted to the fixation of the ova and embryos of Amphibians, is evident to anyone who gives more than casual attention to the matter. The large amount of yolk present in the cells of the Amphibian embryo makes completely uniform fixation with some reagents impossible, and then, too, the disintegrating effect of many fixing fluids upon the yoke granules makes the result very unsatisfactory. A fluid that gives good fixation is likely to interfere with subsequent staining, as witness most of the chromic acid and osmic acid compounds.
Proceedings of the Iowa Academy of Sciences
©1900 Iowa Academy of Science, Inc.
Norris, H. W.
"A Combination of Chromic Acid, Acetic Acid and Formalin as a Fixative for Animal Tissues,"
Proceedings of the Iowa Academy of Science, 8(1), 78-80.
Available at: https://scholarworks.uni.edu/pias/vol8/iss1/10