Spores of Henneguya exilis from the gills of Ictalurus punctatus were exposed to Lugol's solution, Best's carmine, Bauer-Feulgen reaction, and Mitchell's ammoniacal silver nitrate techniques in smears and sections. All of the techniques recognized as specific for glycogen stained the iodinophile vacuole selectively but not specifically, but adjustments to techniques were required. Fresh spores held at room temperatures or chilled for 24 hours were similar insofar as the iodinophile vacuole is concerned, and freezing had little effect. A week or more at room temperature after chilling or freezing for one day resulted in more diffuse staining reactions.
Proceedings of the Iowa Academy of Science
©1969 Iowa Academy of Science, Inc.
Galinsky, Dennis and Meglitsch, Paul A.
"Notes on the Effects of Pretreatment of Spores on the Iodinophile Vacuole of Henneguya exilisl,"
Proceedings of the Iowa Academy of Science, 76(1), 431-435.
Available at: https://scholarworks.uni.edu/pias/vol76/iss1/57