The corollas of L. cardinalis and L. siphilitica were extracted in 1 percent HCl in methanol for 24 hours. This extract (10 ml. portions) was streaked on 3 mm. Whatman filterpaper. The filterpaper was placed in a large chromatographic tank in a solvent system of n-butanol: acetic acid: water (4:1:5 v/v). After separation the bands were cut out and the pigment from each band eluted in a solution of 0.01 percent HCI in methanol. These solutions were then reduced in volume under vacuum and the absorption spectrum of each pigment determined. Aliquots of the crude extract was also spotted on Whatman No. 1 filterpaper and the Rf values of the pigments determined in four different solvent systems. From these data, the main pigment found in L. cardinalis is pelargonidin-3-monoglucoside, and the main pigment in L. siphilitica is a delphinidin-3,5-diglucoside.
Proceedings of the Iowa Academy of Science
©1968 Iowa Academy of Science, Inc.
Wilson, S. Lane
"Anthocyanin Pigments in the Genus Lobelia: I. Pigments in Lobelia Cardinalis L. and L. Siphilitica L.,"
Proceedings of the Iowa Academy of Science, 75(1), 65-68.
Available at: https://scholarworks.uni.edu/pias/vol75/iss1/13