Extensive investigations were conducted on the development of a microbiological assay of a vitamin B6 antagonist found in flaxseed. It is the only known naturally occurring inhibitor of vitamin B6. By means of a screening procedure, ten microorganisms were found to be inhibited by preparations of the B6 antagonist. One of these, Bacillus polymyxa, was investigated more fully. Correlations of the B. polymyxa and live chick assays were excellent. All column chromatographic fractions of inhibitor which reduced Tollen's reagent inhibited B. polymyxa. Vitamin B6 does not competitively reverse the bacterial inhibition. Inhibition of B. polymyxa by high levels of Vitamin B6 and the B6 antagonist may suggest that the antagonist is an analogue of the vitamin.
Proceedings of the Iowa Academy of Science
©1965 Iowa Academy of Science, Inc.
Tjostem, John L.
"Microbiological Studies on the Vitamin B6 Antagonist Found in Flaxseed,"
Proceedings of the Iowa Academy of Science, 72(1), 51-62.
Available at: https://scholarworks.uni.edu/pias/vol72/iss1/12