Inhibition of melanogenesis in larval cells of five amphibian species (two genera) was attained by phenylthiourea treatment. Buff-colored granules were demonstrated in cells of three species, gray granules in two. Pigment returned five to fifteen minutes after withdrawal of PTU. The delay is thought to be due to the time needed for the autocatalytic formation of some dihydroxyphenylalanine, which with tyrosinase is required for melanogenesis, as reported by Lerner and Case. Results obtained with living cells were corroborated by study of fixed material. The data support an origin within the Golgi complex or endoplasmic reticulum.
Proceedings of the Iowa Academy of Science
©1963 Iowa Academy of Science, Inc.
Ladd, Francis Clare
"Return of Pigment in Regenerative Tissue After Inhibition by Phenylthiourea,"
Proceedings of the Iowa Academy of Science, 70(1), 517-526.
Available at: https://scholarworks.uni.edu/pias/vol70/iss1/85