Studies were made to determine the viability of Trichinella spiralis larvae stored in immune and nonimmune swine sera at refrigerator and room temperatures. In trial 1, the trichinae were maintained at room temperature in immune sera for 14 days with only slight reduction in viability, while 7 days storage in nonimmune sera reduced infectivity by nearly 50%. All trichinae were noninfective in both types of sera at 28 days. Room temperature storage in trial 2 gave no loss in viability after 7 days storage in nonimmune sera while infectivity for trichinae stored 7 days in immune sera was 33% of the controls. All infectivity was lost in both sera types after 21 days. Trichinae were maintained at refrigerator temperatures for extended periods in trial 1. Limited infectivity was retained in refrigerated immune sera for 126 days, with 37% viability through 56 days. Trichinae were maintained for 98 days in nonimmune sera, with 50% viability for 56 days. No infectivity was obtained after 28 days storage in either refrigerated serum type during trial 2. Apparent viability as indicated by microscopic examination gave little correlation with actual transmission studies.
Proceedings of the Iowa Academy of Science
© Copyright 1963 by the Iowa Academy of Science, Inc.
Zimmerman, W. J. and Mathews, John
"Viability of Trichinella Spiralis Larvae Stored in Serum,"
Proceedings of the Iowa Academy of Science, 70(1), 479-486.
Available at: https://scholarworks.uni.edu/pias/vol70/iss1/77