The purpose of this study was to determine the relative volume of mitochondria in situ in hepatic cells adjacent to the interlobular veins in the livers of normal A-Jax mice and of A-Jax mice fed carbon tetrachloride dosages for varying periods of time. The liver tissues were fixed in Regaud's fluid, sectioned at 3 microns, and stained with iron haematoxylin. A procedure developed by Meglitsch et al. was utilized in determining the relative mitochondrial volume of the hepatic cells. The following were determined in the study: (1) normal mice older than six weeks possessed a greater relative mitochondrial volume in both the peripheral and basal regions of the median layer liver lobe than six-week normal mice, and (2) the relative mitochondrial volume of hepatic cells of A/Jax mice fed carbon tetrachloride was significantly less than normal mice of similar age.
Proceedings of the Iowa Academy of Science
©1963 Iowa Academy of Science, Inc.
Tone, Jim N. and Johnson, Leland P.
"Relative Mitochondrial Volume in Liver Cells of A/Jax Mice Under Influence of Carbon Tetrachloride,"
Proceedings of the Iowa Academy of Science, 70(1), 458-465.
Available at: https://scholarworks.uni.edu/pias/vol70/iss1/74