Four methods were employed to detect a change in the parenchymal nuclei of liver cells in 8 = week old female A-Jax mice after receiving 600 roentgens of localized X-irradiation. These methods were: (1) determination of the percentage of parenchymal cell volume made up of nuclear material by means of the Chalkley point = ratio method, (2) estimation of the actual nuclear volume by means of camera lucida drawings, (3) estimation of the number of nuclei per cc of tissue, and (4) estimation of the ploidy distribution of the nuclei as inferred from nuclear volumes. Irradiated and non-irradiated mice were sacrificed immediately, in 6 hours, 12 hours, 18 hours, 24 hours, 48 hours, and 72 hours following X-irradiation. No significant difference was noted between the irradiated and between the non-irradiated nuclei from one time of sacrifice to the next. When the data from all times of sacrifice were combined, no significant change attributable to radiation damage was noted in the nuclei.
Proceedings of the Iowa Academy of Science
© Copyright 1963 by the Iowa Academy of Science, Inc.
"The Effects of 600 Roentgens of X-1 Irradiation on the Nuclei of Mouse Liver Cells,"
Proceedings of the Iowa Academy of Science: Vol. 70:
, Article 73.
Available at: https://scholarworks.uni.edu/pias/vol70/iss1/73