Wittman's aceto-iron-haematoxylin stain was combined with Hoyer's mounting medium in a rapid method for demonstrating mitosis in the green algae. Both filamentous and unicellular forms were killed, fixed, and stained in one step with the self-mordanting stain. Cells were then washed with acetic acid and transferred directly to the water-soluble Hoyer's medium. Slides prepared in this manner are permanent. Chlorophyll was bleached from the algal cells by this medium. This revealed the stained nuclei and mitotic figures. This simplified technique is particularly suitable for student use.
Proceedings of the Iowa Academy of Science
©1963 Iowa Academy of Science, Inc.
Bowen, William R.
"The Demonstration of Mitotic Figures in Green Algae,"
Proceedings of the Iowa Academy of Science, 70(1), 138-142.
Available at: https://scholarworks.uni.edu/pias/vol70/iss1/29