Sterilization of potato dextrose agar against bacteria air contaminants occurs during the 6th hour of exposure and against fungi in the 4th hour. The fungicidal activity was broader than the bactericidal activity of propylene oxide. Direct application of propylene oxide to Petri plates containing PDA was ineffective. With plastic plates a chemical reaction took place between the chemical and the plastic. In a closed system sterilization of the plates and medium was accomplished at approximately 1.25 ml of propylene oxide per liter of volume. The addition of propylene oxide directly to nutrient broth effected 90% sterility under certain conditions.
Proceedings of the Iowa Academy of Science
©1963 Iowa Academy of Science, Inc.
Goss, Robert C. and Marr, Jack L.
"Media Sterilization With Propylene Oxide,"
Proceedings of the Iowa Academy of Science, 70(1), 125-129.
Available at: https://scholarworks.uni.edu/pias/vol70/iss1/26