Document Type

Research

Abstract

Embryos of Pinus ponderosa Laws. were excised and placed on a nutrient agar medium to which auxins in varying concentrations had been added. Results indicated that when the auxins were dissolved in and diluted with 40 per cent alcohol there was inhibition of seedling growth and development. Techniques were devised which eliminated the use of alcohol. In the absence of alcohol, morphologically normal seedlings were produced which were transferred to quartz sand after 60 days and at 113 days were transplanted into soil. Apparent normal growth and development continued throughout the duration of the experiment. The addition of auxins produced no significant stimulation to the growth and development of the seedlings.

Publication Date

1962

Journal Title

Proceedings of the Iowa Academy of Science

Volume

Issue

First Page

137

Last Page

142

Copyright

Language

File Format

application/pdf

Recommended Citation

Howe, Virgil K. (1962) "Culture of Excised Embryos of Pinus Ponderosa," Proceedings of the Iowa Academy of Science, 69(1), 137-142.
Available at: https://scholarworks.uni.edu/pias/vol69/iss1/21

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Culture of Excised Embryos of Pinus Ponderosa

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