This paper deals with spectrophotometric and chromatographic analyses of the dye and a study of two staining solutions with a variety of counterstains, using material fixed with ether/ alcohol. These observations indicate that the optimal combination of solutions are 0.5 gm celestine blue B and 40 ml glacial acetic acid heated to 60° C with stirring, to which when completely dissolved 100 ml water and 140 ml 4% ferric alum are slowly added. This should be incorporated with the Papanicolau procedure with the sole omissions of hematoxylin, the acid bath, and the lithium carbonate bath. The result is a very acceptable slide with all the advantages of the Papanicolau technique while employing a nuclear stain that is not hematoxylin, but which has high nuclear specificity. Slides have remained light-fast for at least one year.
Proceedings of the Iowa Academy of Science
©1961 Iowa Academy of Science, Inc.
Dzmura, A. R.; Miroyiannis, S. D.; and Burrows, E. A.
"Celestine Blue B as a Nuclear Stain in Vaginal Exfoliative Cytology,"
Proceedings of the Iowa Academy of Science, 68(1), 586-593.
Available at: https://scholarworks.uni.edu/pias/vol68/iss1/82