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Document Type

Research

Abstract

This paper deals with spectrophotometric and chromatographic analyses of the dye and a study of two staining solutions with a variety of counterstains, using material fixed with ether/ alcohol. These observations indicate that the optimal combination of solutions are 0.5 gm celestine blue B and 40 ml glacial acetic acid heated to 60° C with stirring, to which when completely dissolved 100 ml water and 140 ml 4% ferric alum are slowly added. This should be incorporated with the Papanicolau procedure with the sole omissions of hematoxylin, the acid bath, and the lithium carbonate bath. The result is a very acceptable slide with all the advantages of the Papanicolau technique while employing a nuclear stain that is not hematoxylin, but which has high nuclear specificity. Slides have remained light-fast for at least one year.

Publication Date

1961

Journal Title

Proceedings of the Iowa Academy of Science

Volume

68

Issue

1

First Page

586

Last Page

593

Copyright

© Copyright 1961 by the Iowa Academy of Science, Inc.

Language

EN

File Format

application/pdf

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