Through the coupling of high resolution electron microscopy with the sensitive and flexible techniques of agar diffusion it is possible to study in great detail the nature, site, and degree of intracellular damage caused by exposure to antibody. Tetrahymena is well suited for this type of study since pure cultures are readily grown axenically in the laboratory. Control cells exposed to saline solutions, neutral serum, or heterologous antiserum showed few differences from normal cells. In cells treated with homologous antibody a greatly increased number of cavities appear in the cytoplasm along Pitelka's primary meridian. It is suggested that these cavities are the results of osmotic stress produced by pellicle modification by the antibody, and are therefore not entirely homologous to protrichocysts.
Proceedings of the Iowa Academy of Science
©1961 Iowa Academy of Science, Inc.
Henshaw, Robert E.
"Electron Microscope Observations of Intracellular Responses to Immobilization Antibody in Tetrahymena pyriformis,"
Proceedings of the Iowa Academy of Science, 68(1), 562-580.
Available at: https://scholarworks.uni.edu/pias/vol68/iss1/80