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Authors

Waldo S. Walker

Document Type

Research

Abstract

The highly hydrate condition of the wall material of the cells of collenchyma causes considerable difficulty in the preparation of permanent sections of the material which are suitable for quantitative studies. When standard procedures of dehydration and embedding are employed, excessive shrinkage of the wall material is common. In an attempt to avoid this shrinkage, polyethylene glycol (carbowax) was used as the killing, fixing, dehydrating and embedding medium. This method met with considerable success. The schedule outlined is a modification of that used by A. R. Spurr.

Publication Date

1959

Journal Title

Proceedings of the Iowa Academy of Science

Volume

66

Issue

1

First Page

81

Last Page

85

Copyright

© Copyright 1959 by the Iowa Academy of Science, Inc.

Language

EN

File Format

application/pdf

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