The highly hydrate condition of the wall material of the cells of collenchyma causes considerable difficulty in the preparation of permanent sections of the material which are suitable for quantitative studies. When standard procedures of dehydration and embedding are employed, excessive shrinkage of the wall material is common. In an attempt to avoid this shrinkage, polyethylene glycol (carbowax) was used as the killing, fixing, dehydrating and embedding medium. This method met with considerable success. The schedule outlined is a modification of that used by A. R. Spurr.
Proceedings of the Iowa Academy of Science
©1959 Iowa Academy of Science, Inc.
Walker, Waldo S.
"A Modification of the Carbowax Embedding Technique for Plant Tissue,"
Proceedings of the Iowa Academy of Science, 66(1), 81-85.
Available at: https://scholarworks.uni.edu/pias/vol66/iss1/9