Hawkeye soybean seeds were irradiated with X-rays at dosage levels of 2,500, 5,000, 7,500, 10,000 and 15,000 r. Irradiation had no effect on germination. Plant height 2 weeks after sowing was greatly reduced at 10,000 and 15,000 r., and morphological abnormalities were common. Fertility was greatly reduced at 15,000 r. When seeds were irradiated at dosage levels of 20,000 to 65,000 r., no plant matured and produced seed when dosage was above 35,000 r. The most obvious effect of high dosage X-ray irradiation of seed was the killing of primary leaves and apical meristems. When tested for resistance to Pseudomonas glycinea in the first, second, third and seventh generations after irradiation of seed, progeny from immune plants became increasingly susceptible. When compared in the seventh generation, some plants were less susceptible than others. Resistance to Diaporthe phaseolorum var. caulivora evident in the first generation after irradiation was completely lost by the seventh generation.
Proceedings of the Iowa Academy of Science
©1959 Iowa Academy of Science, Inc.
"X-Ray Irradiation of Soybean Seed as a Technique for Production of Disease Resistant Plants,"
Proceedings of the Iowa Academy of Science, 66(1), 113-122.
Available at: https://scholarworks.uni.edu/pias/vol66/iss1/14