To be of value, the frozen section technique must be simple, fast and must yield slides showing good definition. Most of the methods currently in use are rapid enough but fail to provide clear details; other methods give good definition but are too time-consuming. The method described here is fast, shows good details and results in permanent slides. It makes use of the stain coelestine blue B, employed earlier by Proescher and others (1946) as a nuclear stain in combination with azophloxine as a cytoplasmic stain. The mixture of the two dyes is unstable and has to be made up immediately before use. Gray et al. (1956), state that the staining properties of coelestine blue B would be improved by adjusting its pH to 0.8. At this hydrogen ion concentration the stain exists in the form of a colloid dispersion mixed with a true solution of the unchanged dye. The colloid particles are deposited on the nucleus while the solution of the dye stains the cytoplasm to some extent.
Proceedings of the Iowa Academy of Science
©1958 Iowa Academy of Science, Inc.
Dzmura, Robert and Gray, Peter
"Rapid Nuclear Staining Method for Frozen Sections,"
Proceedings of the Iowa Academy of Science, 65(1), 450-454.
Available at: https://scholarworks.uni.edu/pias/vol65/iss1/68