Embryonic Cell Respiration: The Effect of Methylene Blue and 2-4 Dinitrophenol on Embryonic Cell Respiration

Authors

Joseph Hall Bodine, State University of IowaFollow
Laurence Rockwell Fitzgerald, State University of IowaFollow

Document Type

Research

Abstract

The effects of M.b. and 2-4 D.N.P. on the oxygen uptake of mitotically active and blocked grasshopper embryos have been worked out over wide concentration ranges. For both reagents an increase in stimulating effects occurs with increasing concentrations up to a maximum. For M.b. this maximum stimulation continues over a very wide range of concentrations and little injury for exposures of 1 to 2 hours results. With 2-4 D.N.P. rather marked toxic effects occur after the maximum stimulating doses are increased. The relative efficiency of the two reagents is such that 2-4 D.N.P. exerts greater stimulating effects than M.b. at similar concentrations. No evidence has been obtained to indicate any additive effects of the reagents on the oxygen uptake when applied to the cell. Once maximum stimulation has been produced by either compound further additions of stimulating doses of either reagent do not change the result.

Publication Date

1949

Journal Title

Proceedings of the Iowa Academy of Science

Volume

56

Issue

1

First Page

311

Last Page

321

Copyright

©1949 Iowa Academy of Science, Inc.

Language

en

File Format

application/pdf

Recommended Citation

Bodine, Joseph Hall and Fitzgerald, Laurence Rockwell (1949) "Embryonic Cell Respiration: The Effect of Methylene Blue and 2-4 Dinitrophenol on Embryonic Cell Respiration," Proceedings of the Iowa Academy of Science, 56(1), 311-321.
Available at: https://scholarworks.uni.edu/pias/vol56/iss1/44