Sudan IV is commonly used to stain fats, oils, suberin, and cutin. Materials stained in this dye are usually mounted temporarily in glycerine and are seldom kept as permanent slides. This may be due to the fact that balsam, clarite or similar mounting media, cannot be used to make permanent slides of preparations stained in Sudan IV. The dye is immediately removed by the xylene or toulene solvent of these media, leaving the preparations colorless.
Proceedings of the Iowa Academy of Science
©1942 Iowa Academy of Science, Inc.
Dean, H. L. and Sybil, Edwards Jr.
"Permanent Slides of Plant Cuticle Stained with Sudan IV and Sudan Black B,"
Proceedings of the Iowa Academy of Science, 49(1), 129-132.
Available at: https://scholarworks.uni.edu/pias/vol49/iss1/14