Colchicine injected into the body cavity of insects affects the mitotic process of the germ cells by destroying the spindle. The chromosomes become clumped or scattered depending upon the concentration of the colchicine used. The injections were of .OS5cc. of concentrations ranging from .5 per cent — .O5 per cent solutions. A testis from an animal injected with colchicine often shows numerous giant spermatids. Accompanying this karyokinetic disturbance the mitochondria, which are generally in the form of threads along the spindle fibers during the metaphase and anaphase, fail to assume such arrangement. They are found diffused in the cytoplasm in the form of short rods and granules, instead of the usual threads. The mitochondria affected in a dividing cell may assume an irregular mass in the daughter cells. The Golgi bodies seem to be less affected showing only a slight swelling.
Proceedings of the Iowa Academy of Science
©1939 Iowa Academy of Science, Inc.
Dooley, Thomas P.
"The Influence of Colchicine on the Germ Cells of Insects, Melanoplus Differentialis and Gryllus Assimilis, With Special Reference to the Cytoplasmic Inclusions,"
Proceedings of the Iowa Academy of Science, 46(1), 445-446.
Available at: https://scholarworks.uni.edu/pias/vol46/iss1/133