Variations in concentration of tyrosinase throughout the entire embryonic development of the grasshopper, Melanoplus differentialis, have been determined by measuring the oxygen uptake of the tyrosinase-tyrosine reaction with the Barcroft-Warburg apparatus. Tyrosinase activity of eggs at different developmental stages has thus been expressed as the amount of O2 consumed per 100 minute interval at 25 ° C. in the oxidation of a given amount of tyrosine by the enzyme extracted (in phosphate buffer pH 8.0) from 20 eggs.
Proceedings of the Iowa Academy of Science
©1935 Iowa Academy of Science, Inc.
Bodine, J. H. and Boell, E. J.
"Enzymes in Ontogenesis (orthoptera): I. Tyrosinase,"
Proceedings of the Iowa Academy of Science, 42(1), 210-211.
Available at: https://scholarworks.uni.edu/pias/vol42/iss1/102