One of the best methods for the determination of manganese in very small amounts in biological material is the adaptation of Willard's oxidation of the Mn in acid solution by KIO4 , and a comparison in Nessler tubes of the color produced, with a known standard similarly treated. Skinner and Peterson showed that if animal tissue was ashed in a muffle furnace and extracted with H2PO4 , the KIO4 oxidized the Mn in the solution without loss of color. They found 0.01 mg Mn in 50 cc. solution gave a readable color in Nessler tubes against an inverted V of white paper.
Proceedings of the Iowa Academy of Science
©1933 Iowa Academy of Science, Inc.
Clark, Norman Ashwell
"The Determination of Manganese in Biological Material,"
Proceedings of the Iowa Academy of Science, 40(1), 113-114.
Available at: https://scholarworks.uni.edu/pias/vol40/iss1/55