The formation of acetyl-methyl-carbinol by members of the genus Aerobacter was detected in six-hour cultures. The procedure involved the heavy inoculation of young agar slant cultures into approximately three cc. Difeo M.R.-V.P. medium, incubation at 30° C. for six hours and using 0.3 per cent creatine in 40 per cent KOH as the test reagent. Seventy per cent of 202 cultures tested produced positive results within one hour after the addition of the test reagent and over ninety-nine per cent within four hours. The creatine KOH solution was satisfactorily effective for three weeks when kept at ordinary laboratory conditions and for six weeks when stored at ice-box temperatures.
Proceedings of the Iowa Academy of Science
©1933 Iowa Academy of Science, Inc.
Epstein, S. S. and Levine, Max
"Some Notes on the Voges-Proskauer Reaction,"
Proceedings of the Iowa Academy of Science, 40(1), 80-80.
Available at: https://scholarworks.uni.edu/pias/vol40/iss1/23