•  
  •  
 

Document Type

Research

Abstract

The alpha-naphthylamine-sulphanilic acid test is widely employed for the detection of reduction of nitrates to nitrites by bacteria. The test has the disadvantage that the reel coloration produced in the presence of the nitrite ion appears for only a few seconds and then fades in cultures of organisms in which a relatively high concentration of nitrite occurs. Frequently in our work it is necessary to test considerable numbers of cultures at one time. Fading makes the results unreliable and cultures having a relatively high concentration of nitrite may be recorded as negative. A second difficulty experienced with the test is the instability of the alpha-naphthylamine solution, resulting in its marked discoloration. When added to the culture, the discolored solution imparts a pink color which may prove confusing when testing cultures having relatively little nitrite present.

Publication Date

1930

Journal Title

Proceedings of the Iowa Academy of Science

Volume

37

Issue

1

First Page

53

Last Page

55

Copyright

© Copyright 1930 by the Iowa Academy of Science, Inc.

Language

EN

File Format

application/pdf

Share

COinS
 
 

To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.