The first of the two following experiments was performed in 1927-1928. Thirty-five sexually mature earthworms (Lumbricidae) were put under the influence of ether. Anesthesia was resorted to so that the subjects would remain inactive during the subsequent operation. A tiny incision was made in the ventral body wall of each specimen, at a point just posterior to the clitellum. The ventral nerve cord was then severed with a fine pointed scissors. It will be noticed that the place of operation was posterior to the more important organs of the body. It was selected as such for the foregoing reason and also because the clitellum furnished the only available spot that might readily be identified at or after the time of operation.
Proceedings of the Iowa Academy of Science
©1929 Iowa Academy of Science, Inc.
Fitzpatrick, F. L. and Stolba, S. L.
"Segmentation in Earthworms, Resulting from Inability to Regenerate a Portion of the Ventral Nerve Cord,"
Proceedings of the Iowa Academy of Science, 36(1), 361-363.
Available at: https://scholarworks.uni.edu/pias/vol36/iss1/112