Beijerinck in 1911 called attention to the fact that some strains of Bact. aaerogenes produce from chinic acid and a ferric salt, a red to black coloration under aerobic conditions. Strains of Bact. coli, he stated, do not color the medium under similar conditions. Under anaerobic conditions in solutions of chinate salt, a fermentation may occur with some members of the aerogenes group, whereby carbonic, acetic and propionic acids are formed. At the Iowa State College laboratory an attempt was made to duplicate this anaerobic type of fermentation by organisms of the coli-aerogenes group but without success. Both Smith and Durham tubes were used and no gas was detected. However, the coloration produced by Bact. aerogenes when grown on chinate medium was found to be pronounced. This color is soluble and diffuses through the medium, either liquid or solid. The suggestion from this article of Beijerinck's presented an opportunity for the development of a possible differential test for coli and aerogenes strains.
Proceedings of the Iowa Academy of Science
©1924 Iowa Academy of Science, Inc.
Butcher, Buford H.
"Utilization of Chinic Acid in the Differentiation of the Colon-Aerogenes Groups,"
Proceedings of the Iowa Academy of Science, 31(1), 97-100.
Available at: https://scholarworks.uni.edu/pias/vol31/iss1/10