In the preparation of amphibian eggs and larvae for histological study, the technique employed is a factor of primary importance. The amphibian egg represents the final attempt on the part of nature to produce a large yolk-laden egg in which cleavage involves the entire ovum. As a result of this method of cleavage all the embryonic tissues, during the early stages of development, contain more or less yolk. Furthermore, the ventral region of the young embryo contains a large, compact yolk-mass which is not readily penetrated by the fixing and the clearing agent, and the embryonic tissues show a greater tendency to shrink than do the tissues of other vertebrate embryos.
Proceedings of the Iowa Academy of Science
©1911 Iowa Academy of Science, Inc.
"Notes on Methods for the Study of Amphibian Eggs and Larvae,"
Proceedings of the Iowa Academy of Science, 18(1), 145-146.
Available at: https://scholarworks.uni.edu/pias/vol18/iss1/30