Honors Program Thesis (UNI Access Only)
James Jurgenson, Honors Thesis Advisor
Researchers make use of this system by creating RNA that will bind to both the DNA of the target cell and an enzyme (usually Cas9). This causes DNA to be cut at the location that was targeted. When a DNA cut such as this occurs, the cell naturally repairs the area by adding genetic material. The guide RNA, which was attached to the now-cut DNA, has the changes to genetic material desired by researchers coded into it. As the cell repairs itself, it creates new DNA that incorporates these changes. Using this system, researchers are able to induce genetic changes in organisms much more efficiently than with other techniques.
Year of Submission
Department of Biology
University Honors Designation
A thesis submitted in partial fulfillment of the requirements for the designation University Honors
1 PDF file (24 pages)
©2020 Jacob Horn
Horn, Jacob, "Preparation of the CRISPR Cas-9 system for directed mutagenesis of the FSR1 gene of the filamentous fungi Fusarium verticillioides" (2020). Honors Program Theses. 437.