Assay Of Plant Proteins With Bicinchoninic Acid For High Resolution Two-Dimensional Polyacrylamide Gel Electrophoresis

Authors

Alan R. Orr, University of Northern IowaFollow
Brett A. Wagner, University of Northern Iowa
Catherine T. Howard, University of Northern Iowa
Orlando A. Schwartz, University of Northern IowaFollow

Document Type

Article

Journal/Book/Conference Title

Plant Cell Reports

Volume

7

Issue

7

First Page

598

Last Page

601

Abstract

amethod is described for estimating proteins in the same plant tissue sample that is solubilized for separation by two-dimensional polyacrylamide gel electrophoresis. The method uses amodified bicinchoninic acid (BCA) protein assay procedure and amodified standard ureasolubilization buffer to estimate microgram values of unknown protein concentration, in the presence of 9 M ureaand 4% Nonidet P-40, from alinear standard curve. amethod for aquantitative determination of protein concentration by BCain asample containing 9 M ureaand 4% Nonidet P-40 is also described. This method is effective for the determination of proteins in minute non-green and green plant tissue, and is especially designed for vegetative and floral shoot apices, and the primordiaof inflorescences. © 1988 Springer-Verlag.

Department

Department of Biology

Original Publication Date

12-1-1988

DOI of published version

10.1007/BF00272767

Recommended Citation

Orr, Alan R.; Wagner, Brett A.; Howard, Catherine T.; and Schwartz, Orlando A., "Assay Of Plant Proteins With Bicinchoninic Acid For High Resolution Two-Dimensional Polyacrylamide Gel Electrophoresis" (1988). Faculty Publications. 4662.
https://scholarworks.uni.edu/facpub/4662