The biodegradation potential of vegetable-based hydraulic fluids using a miniaturized test method
Resource and Environmental Biotechnology
The concern of environmental contamination through use and misuse of hydrocarbon-based lubricants has encouraged the development of alternative materials. Environmentally acceptable lubricants (EAL) should be both non-toxic and biodegradable. According to regulatory agencies, a test material is considered readily biodegradable if the material has been converted into more than 60% of the theoretical CO2 production after 28 days in a bioassay system. We describe a miniaturized bioassay which measures CO2 production from a soybean oil-based hydraulic fluid by determining the remaining hydroxide in a Ba(OH)2 solution (trap) in which produced CO2 is dissolved. Bacteria from soil were grown in enrichments containing soybean oil-based hydraulic fluid, isolated, cultured, identified and then used as inoculum to test this method. Biodegradation results show that the soybean oil-based lubricant tested is rapidly biodegradable. The results also demonstrated that a miniaturized method that is inexpensive and minimizes chemical wastes can be used for measuring biodegradation potentials.
Original Publication Date
Rouzic, Lionel J.; Mascarenhas, Terumi; and Brown, Edward J., "The biodegradation potential of vegetable-based hydraulic fluids using a miniaturized test method" (2000). Faculty Publications. 3703.