Faculty Publications

Title

Characterization of Gene Repression by Designed Transcription Activator-like Effector Dimer Proteins

Document Type

Article

Journal/Book/Conference Title

Biophysical Journal

Volume

119

Issue

10

First Page

2045

Last Page

2054

Abstract

Gene regulation by control of transcription initiation is a fundamental property of living cells. Much of our understanding of gene repression originated from studies of the Escherichia coli lac operon switch, in which DNA looping plays an essential role. To validate and generalize principles from lac for practical applications, we previously described artificial DNA looping driven by designed transcription activator-like effector dimer (TALED) proteins. Because TALE monomers bind the idealized symmetrical lac operator sequence in two orientations, our prior studies detected repression due to multiple DNA loops. We now quantitatively characterize gene repression in living E. coli by a collection of individual TALED loops with systematic loop length variation. Fitting of a thermodynamic model allows unequivocal demonstration of looping and comparison of the engineered TALED repression system with the natural lac repressor system.

Department

Department of Chemistry and Biochemistry

Original Publication Date

11-17-2020

DOI of published version

10.1016/j.bpj.2020.10.007

Repository

UNI ScholarWorks, Rod Library, University of Northern Iowa

Language

en

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